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rabbit polyclonal anti e2f2  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology rabbit polyclonal anti e2f2
    Rabbit Polyclonal Anti E2f2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 855 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+e2f2/pm27802335-94-15-41?v=Santa+Cruz+Biotechnology
    Average 95 stars, based on 855 article reviews
    rabbit polyclonal anti e2f2 - by Bioz Stars, 2026-07
    95/100 stars

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    rabbit anti-human e2f2 polyclonal antibody sc-632  (Santa Cruz Biotechnology)
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    Santa Cruz Biotechnology rabbit anti-human e2f2 polyclonal antibody sc-632
    <t>E2F2</t> is a direct target of miR-218. (A) Predicted binding sites for the seed sequences of miR-218 and miR-520a in the E2F2 3′-UTR and the sites of target mutagenesis are presented. (B) A dual luciferase assay was conducted in order to confirm the direct regulation of miR-218 on the E2F2 3′-UTR. miR-218 and miR-520a regulate E2F2 expression at the (C) mRNA and (D) protein levels in Huh7 and MHCC-97H cells. Values are presented as the mean ± standard deviation (n=3). * P<0.05, ** P<0.01, vs. NC. miR-218, microRNA-218; UTR, untranslated region; NC, negative control; Wt, wild-type; mt, mutant.
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    Table S3.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: An NAD + -dependent transcriptional program governs self-renewal and radiation resistance in glioblastoma

    doi: 10.1073/pnas.1610921114

    Figure Lengend Snippet: Table S3.

    Article Snippet: The following antibodies were used for this study: rabbit polyclonal anti-Visfatin (ab45890; Abcam), rabbit polyclonal anti-PBEF (A300-372A; Bethyl Laboratories), mouse monoclonal anti- IDH1 R132H (DIA-H09-L; HistoBioTec), rabbit polyclonal anti-E2F2 (sc-633; Santa Cruz Biotechnology, Inc.), rabbit monoclonal anti-Id1 (BCH-1/195-14; BioCheck, Inc.), mouse monoclonal anti-α-tubulin (T5168; Sigma-Aldrich), mouse monoclonal anti-FLAG (F3165; Sigma-Aldrich), rabbit polyclonal anti-GFP (A6455; Life Technologies), and rabbit Anti-Phospho-Histone H2A.X (Ser139) (2577; Cell Signaling).

    Techniques:

    E2F2 is a direct target of miR-218. (A) Predicted binding sites for the seed sequences of miR-218 and miR-520a in the E2F2 3′-UTR and the sites of target mutagenesis are presented. (B) A dual luciferase assay was conducted in order to confirm the direct regulation of miR-218 on the E2F2 3′-UTR. miR-218 and miR-520a regulate E2F2 expression at the (C) mRNA and (D) protein levels in Huh7 and MHCC-97H cells. Values are presented as the mean ± standard deviation (n=3). * P<0.05, ** P<0.01, vs. NC. miR-218, microRNA-218; UTR, untranslated region; NC, negative control; Wt, wild-type; mt, mutant.

    Journal: Molecular Medicine Reports

    Article Title: MicroRNA-218 and microRNA-520a inhibit cell proliferation by downregulating E2F2 in hepatocellular carcinoma

    doi: 10.3892/mmr.2015.3516

    Figure Lengend Snippet: E2F2 is a direct target of miR-218. (A) Predicted binding sites for the seed sequences of miR-218 and miR-520a in the E2F2 3′-UTR and the sites of target mutagenesis are presented. (B) A dual luciferase assay was conducted in order to confirm the direct regulation of miR-218 on the E2F2 3′-UTR. miR-218 and miR-520a regulate E2F2 expression at the (C) mRNA and (D) protein levels in Huh7 and MHCC-97H cells. Values are presented as the mean ± standard deviation (n=3). * P<0.05, ** P<0.01, vs. NC. miR-218, microRNA-218; UTR, untranslated region; NC, negative control; Wt, wild-type; mt, mutant.

    Article Snippet: Following blocking with 5% skimmed milk, the membranes were incubated with rabbit anti-human E2F2 polyclonal antibody (1:200; sc-632; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) overnight at 4°C.

    Techniques: Binding Assay, Mutagenesis, Luciferase, Expressing, Standard Deviation, Negative Control